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mouse anti glp 1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti glp 1
    Mouse Anti Glp 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+glp+1r/GLP-1R+Antibody/pm41508004-133-16-12
    Average 94 stars, based on 136 article reviews
    mouse anti glp 1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Activation of microglial GLP-1R in the trigeminal nucleus caudalis suppresses central sensitization of chronic migraine after recurrent nitroglycerin stimulation
    Article Snippet: .. Then, the blots were incubated with the following antibodies overnight at 4 °C: mouse anti-GLP-1R (1:200, Santa Cruz, CA, USA), mouse anti-GLP-1 (1:100, Santa Cruz, CA, USA), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), mouse anti-c-fos (1:200, Santa Cruz, CA, USA), rabbit anti-Iba-1 (1:1000, Abcam, Cambridge, MA, USA), rabbit anti-PI3K (1:1000, Proteintech, China), rabbit anti-Akt (1:1000, Proteintech, China), rabbit anti-phospho-Akt (1:2000, Cell Signalling, Boston, MA, USA), rabbit anti-IL-1β (1:800, Wanleibio, China), rabbit anti-TNF-α (1:800, Wanleibio, China), and mouse anti-β-actin (1:1000, Beyotime, China). .. After the membranes had been washed with TBST, the secondary antibodies (goat anti-rabbit, 1:1000; goat anti-mouse, 1:1000; Beyotime, China) were applied and incubated for 1 h at RT.

    Article Title: Activation of microglial GLP-1R in the trigeminal nucleus caudalis suppresses central sensitization of chronic migraine after recurrent nitroglycerin stimulation
    Article Snippet: The whole brain and cervical spinal cord (C1-C2) were collected and postfixed with 4 % PFA overnight at 4 °C.The medullary segment including the TNC between + 1 and − 3 mm from the obex was removed and transferred to 30 % sucrose for 48 h. After being frozen at − 80 °C, samples were cut into 10-μm-thick sections on a cryostat (Thermo). .. Sample sections were blocked in 5 % goat serum with 0.3 % Triton X-100 for 1 h at RT and then incubated overnight at 4 °C with the following primary antibodies: mouse anti-GLP-1R (1:50, Santa Cruz, CA, USA), mouse anti-GLP-1(1:50, Santa Cruz, CA, USA), rabbit anti-Iba1 (1:500, Wako Chemicals, Tokyo, Japan), rabbit anti-GFAP (1:200, Cell Signalling, Boston, MA, USA), rabbit anti-NeuN (1:100, Proteintech, China), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), and mouse anti-c-fos (1:100, Santa Cruz, CA, USA). .. The sections were then incubated with fluorescence-conjugated secondary antibodies (Alexa Fluor Cy3 and Alexa Fluor 488, 1:500, Beyotime, China).

    Article Title: Acupuncture ameliorates diet-induced obesity via the vagal–GLP-1–ARC circuit: neural mechanism of anorexigenic action
    Article Snippet: After washed with TBST, sections were blocked with 10% goat/donkey serum (37 °C, 1h), incubated overnight at 4 °C with primary antibodies: rabbit anti-POMC (1:1000, ab210605, Abcam, USA), rabbit anti-NPY (1:1000, ab221145, Abcam, USA) or rabbit anti-c-fos (1:800, #2250, CST, USA), followed by secondary antibodies donkey anti-rabbit Alexa Fluor® 594 (1:500, ab150064, Abcam, USA) or goat anti-rabbit CoraLite594 (1:800, SA00013-4, Proteintech, China). .. For dual labeling, sections were re-blocked, incubated with mouse anti-GLP-1R (1:100, SC-390773, Santa Cruz, USA) or mouse anti-GLP-1 (1:200, #BF8043, Affinity, USA) and stained with secondary antibodies goat anti-mouse CoraLite488 (1:200, SA00013-1, Proteintech, China). .. Nuclei were counterstained with DAPI (Southern Biotech, China, 0100–20).

    Labeling:

    Article Title: Acupuncture ameliorates diet-induced obesity via the vagal–GLP-1–ARC circuit: neural mechanism of anorexigenic action
    Article Snippet: After washed with TBST, sections were blocked with 10% goat/donkey serum (37 °C, 1h), incubated overnight at 4 °C with primary antibodies: rabbit anti-POMC (1:1000, ab210605, Abcam, USA), rabbit anti-NPY (1:1000, ab221145, Abcam, USA) or rabbit anti-c-fos (1:800, #2250, CST, USA), followed by secondary antibodies donkey anti-rabbit Alexa Fluor® 594 (1:500, ab150064, Abcam, USA) or goat anti-rabbit CoraLite594 (1:800, SA00013-4, Proteintech, China). .. For dual labeling, sections were re-blocked, incubated with mouse anti-GLP-1R (1:100, SC-390773, Santa Cruz, USA) or mouse anti-GLP-1 (1:200, #BF8043, Affinity, USA) and stained with secondary antibodies goat anti-mouse CoraLite488 (1:200, SA00013-1, Proteintech, China). .. Nuclei were counterstained with DAPI (Southern Biotech, China, 0100–20).

    Staining:

    Article Title: Acupuncture ameliorates diet-induced obesity via the vagal–GLP-1–ARC circuit: neural mechanism of anorexigenic action
    Article Snippet: After washed with TBST, sections were blocked with 10% goat/donkey serum (37 °C, 1h), incubated overnight at 4 °C with primary antibodies: rabbit anti-POMC (1:1000, ab210605, Abcam, USA), rabbit anti-NPY (1:1000, ab221145, Abcam, USA) or rabbit anti-c-fos (1:800, #2250, CST, USA), followed by secondary antibodies donkey anti-rabbit Alexa Fluor® 594 (1:500, ab150064, Abcam, USA) or goat anti-rabbit CoraLite594 (1:800, SA00013-4, Proteintech, China). .. For dual labeling, sections were re-blocked, incubated with mouse anti-GLP-1R (1:100, SC-390773, Santa Cruz, USA) or mouse anti-GLP-1 (1:200, #BF8043, Affinity, USA) and stained with secondary antibodies goat anti-mouse CoraLite488 (1:200, SA00013-1, Proteintech, China). .. Nuclei were counterstained with DAPI (Southern Biotech, China, 0100–20).



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